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(A) Lysates from 293T cells overexpressing FLAG-SRPK1 or FLAG-SRPK1Δspacer were analyzed by SDS-PAGE, under non-reducing or reducing conditions, and Western blotting. SRPK1 was detected using the M5 anti-FLAG monoclonal antibody. (B) Amino acid sequence of SRPK1. The two catalytic domains are highlighted by grey shadows. All cysteines are marked in red; underlined cysteines were mutated to alanine or glycine.
