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. Author manuscript; available in PMC: 2017 Feb 6.
Published in final edited form as: Bio Protoc. 2016 Nov 5;6(21):e1998. doi: 10.21769/BioProtoc.1998

Table 3.

PCR cycling conditions

First PCRs Second PCRs
1 x 30 sec 98 °C 1 x 30 sec 98 °C
10 x 15 sec 98 °C 10 x 15 sec 98 °C
30 sec 59-54.5 °C (− 0.5 °C/cycle) 30 sec 59-54.5 °C (− 0.5 °C/cycle)
90 sec 72 °C 60 sec 72 °C
30 x 15 sec 98 °C 30 x 15 sec 98 °C
30 sec 54 °C 30 sec 54 °C
90 sec 72 °C 60 sec 72 °C
1 x 3 min 72 °C 1 x 3 min 72 °C