. 2017 Feb 6;12(2):e0171126. doi: 10.1371/journal.pone.0171126

Table 2. Stratified analysis of parasitaemia comparing DBS and WB HTP-LAMP against nPCR with a cut-off at 2 p/μL.

*Parasitaemia >2 p/μL*	PCR(+)	PCR(-)	Sensitivity(95% CI)	Specificity(95% CI)	PPV, %	NPV,%	Parasites/μL^a (Geometric mean)	Plasmodium species distribution
DBS-HTP-LAMP vs. nPCR n = 688
DBS HTP-LAMP (+)	60	0	100	100	100	100	2.0–912896.0	n = 57, P. falciparum from ≥ 4 p/μL
DBS HTP-LAMP (-)	0	628					(711.5)	n = 2, P. vivax each at 2 p/μL;n = 1, P. ovale at 2 p/μL
WB-HTP-LAMP vs. nPCR n = 688
WB-HTP-LAMP (+)	60	0	100	100	100	100	2.0–912896.0	n = 57, P. falciparum from ≥ 4 p/μL
WB-HTP-LAMP (-)	0	628					(711.5)	n = 2, P. vivax each at 2 p/μL;n = 1, P. ovale at 2 p/μL
*Parasitaemia < 2p/μL*	PCR (+)	PCR (-)	Sensitivity(95% CI)	Specificity (95% CI)	PPV, %	NPV, %	Parasites/μL^a (Geometric mean)	Plasmodium species distribution
DBS-HTP-LAMP vs. nPCR n = 639
DBS HTP-LAMP (+)	7	0	77.8	100	100	99.7	undetected-1.5	n = 5, P. falciparum from 0.5–1.5 p/μL
DBS HTP-LAMP (-)	2	630	(54.3–100)				(1.03)	n = 2, P. falciparum, P. ovale (undetected by qPCR^b, DBS positive) n = 1, P. falciparum at 0.8 p/μL,
								(DBS negative)
								n = 1, P. falciparum (undetected by qPCR^b; DBS negative)
WB-HTP-LAMP vs. nPCR n = 639
WB-HTP-LAMP (+)	8	2	88.9	99.7	80	99.8	undetected-1.5	n = 5, P. falciparum from 0.5–1.5 p/μL
WB-HTP-LAMP (-)	1	628	(71.1–100)	(99.2–100)			(1.03)	n = 2, P. falciparum (undetected by qPCR^b, WB positive) n = 1, P. ovale (undetected by qPCR^b, WB positive)n = 1, P. falciparum at 0.8 p/μL, (WB negative)

^a Range of parasite densities for PCR positive results

^b Parasite density could not be quantified due to negative qPCR results.