Fig 3. Blocked initiation leads to loss of quarter cell foci.

DnaN-YPet (in E. coli) is imaged in both in wild type and cells containing the dnaC2 allele at 37°C. Under these conditions, cells containing the mutant allele will be blocked from initiating new rounds of replication. A: Example wild type cell towers showing the the disappearance of the midcell focus may be followed appearance of a pair of foci near the quarter-cell positions. B: Conditional probability distribution (N = 4837 time points) shows localizations near the quarter cell positions in the wild type near the end of the cell cycle. C: Example cell towers for cells with blocked initiation do not show foci at the quarter-cell positions after disappearance of the mid-cell focus. D) In cells blocked for initiation, conditional probability no longer shows a significant number of localization at the quarter-cell positions (N = 1758 time points).