Figure 9.

Early apoptosis validation.

Notes: MCF7 cells were treated with IC₅₀ of 8-hydroxy-ar-turmerone for 48 h. Cells were incubated for 20 min with Annexin V-FITC and the buffer. Untreated cells showed viable cells with no signs of apoptosis (A). Light green stained with Annexin V-FITC revealed that PS residues had been translocated to the membrane and externalized (phosphatidylserine) after treatment. Treated cells are shown with (B) 20× and (C) 40× magnification.

Abbreviations: FITC, fluorescein isothiocyanate; IC₅₀, half maximal inhibitory concentration.