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Effect of UDP-Xyl on Hysteresis
| Enzyme | UDP-Xyl (μM) | τ (s−1) | Lag (s) | νi | νss |
|---|---|---|---|---|---|
| hUGDH | 0 | 16.3 ± 0.24 | 44.3 ± 0.66 | 0.43 ± 0.02a | 1.0 ± 0.01a |
| 3b | 38.3 ± 2.7 | 104 ± 7.3 | 0.35 ± 0.03 | 1.1 ± 0.01 | |
| hUGDHA136M | 0 | N.D.c | N.D. | N.D. | 1.3 ± 0.03 |
| 60b | N.D. | N.D. | N.D. | 1.4 ± 0.03 |
units = nM NADH per nM enzyme per second.
These concentrations of UDP-Xyl are ≥10× the Ki listed in Table 4
N.D.= not detected
