. Author manuscript; available in PMC: 2018 Jan 10.

Published in final edited form as: Biochemistry. 2016 Dec 22;56(1):202–211. doi: 10.1021/acs.biochem.6b01044

Table 3.

Steady State Parameters

Enzyme	Ligand	KM (μM)	Hill	k_cat (s⁻¹)^a	K₀^b	K_lim^b	k_cat^b
hUGDH	UDP-Glc	9.7±0.8	1	0.55±0.01
	NAD+	780±180	0.73 ± 0.07	0.76±0.05	88 ± 20	1800 ± 400	0.79± 0.06
	UDP-Glc	7.26±0.67	1	0.73±0.02
hUGDH_A136M	NAD+	90±6	1	0.83±0.02

One complete catalytic turnover produces 2 NADH,

results from Kurganov analysis of negatively cooperative enzyme