Video 13. Muscle progenitors arise in the piwi-1+ blastomere population during S5.
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DOI: 10.7554/eLife.21052.057
SPIM reconstructed S5 embryo costained with piwi-1 and VAL-like (both in red) and myoD (green). Muscle progenitors, coexpressing piwi-1 and myoD, are dispersed in the embryonic wall. As muscle progenitors differentiate, they are predicted to downregulate piwi-1 and to retain expression of myoD. VAL-like is expressed the temporary embryonic pharynx and is not detected in piwi-1+ blastomeres.