Figure 2. Coupled PRODH–P5CDH activity of EcRHH–RcPutA.

The assay was performed using 200 μM CoQ₁, 40 mM proline, 0.25 μM EcRHH–RcPutA in 50 mM potassium phosphate (pH 7.5) and 25 mM NaCl. NADH formation was monitored at 340 nm. For comparison, a trace of RcPutA activity is also shown from a channelling assay performed under identical conditions [29].