Table 1. PRODH kinetic parameters for EcRHH–RcPutA and RcPutA.
Proline | CoQ1 | |||||
---|---|---|---|---|---|---|
Enzyme | Km (mM) | kcat (s−1) | kcat/Km (M−1 s−1) | Km (μM) | kcat (s−1) | kcat/Km (M−1 s−1) |
EcRHH–RcPutA | 5.3±0.9 | 0.89±0.1 | 167.9±5.2 | 161.0±15.8 | 1.1±0.1 | 6832±715 |
RcPutA | 5.6±0.8 | 1.0±0.1 | 178.6±30 | 93.7±19.0 | 2.0±0.1 | 21505±4579 |
Kinetic parameters were determined in 50 mM potassium phosphate, 25 mM NaCl, pH 7.5. Data obtained from Luo et al. [29].