Skip to main content
. 2016 Apr 15;36(2):e00318. doi: 10.1042/BSR20160054

Figure 4. Effect of ATP and Ra2 co-culture on the barrier function of MBECs.

Figure 4

(A) TEER measurements of medium only (blank), an MBEC monolayer grown on gold-film electrodes (control) and MBECs cultured with 100 μM ATP (ATP), 100 μM UTP (UTP), Ra2 cells with 100 μM ATP (Ra2 ATP) or RAW264.7 cells with 100 μM ATP (RAW264.7 ATP) (0 min). (B) Effect of Ra2 or RAW264.7 cell co-culture in the presence of ATP on the barrier function of the MBEC monolayer as evaluated by cell–cell contact resistance (Rb) and cell–matrix adhesion impedance (α). Normalized TEER (Normal TEER) values and normal ECIS parameters are presented as the mean±S.E.M. for three or four experiments; *, P<0.05 (two-tailed Student's t test).