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. 2016 May 20;36(3):e00336. doi: 10.1042/BSR20160088

Figure 5. Manipulation of HOTAIR expression affects Sirt1 expression.

Figure 5

(A) MS-PCR was used to evaluate promotor methylation level of Sirt1 in HepG2 cells transfected with pcDNA-HOTAIR. (B) Relative expression of HOTAIR was determined in pcDNA-HOTAIR-transfected HepGs cells. (C) Analysis of Sirt1 mRNA and protein expression in HepG2 cells transfected with pcDNA-HOTAIR. (D) Relative expression of HOTAIR in si-HOTAIR-transfected HepG2 cell. (E) Analysis of Sirt1 mRNA and protein expression. **P<0.01 compared with cells transfected with empty pcDNA vector, or si-control.