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. 2016 Jun 30;36(3):e00354. doi: 10.1042/BSR20150300

Figure 3. miR-148b directly targets the 3’-UTR of MLH1 and regulate its expression level in lung cancer cells.

Figure 3

(A) The wild-type 3’-UTR of mammalian MLH1 mRNA contains a putative miR-148b-binding site. The mutant form was shown below. (B and C) Luciferase reporter assays. A549 cells were transfected with reporters containing the wild-type or mutant form after transfection with control miRNA as well as miR-148b mimic or inhibitor respectively. Data represent the mean ± S.D. *, P<0.05; **, P<0.01. (D) Western Blot analysis of MLH1 protein level in A549 cells transfected with control, miR-148b mimic or inhibitor. β-Actin was used as loading control.