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. 2016 Jun 3;36(3):e00343. doi: 10.1042/BSR20160123

Figure 3. Simulation of HEY1 phosphorylation at residue Ser-68 specifically alters HEY1 protein–protein interactions.

Figure 3

(A) Whole-cell extracts from U2OS cells previously transfected with expression vectors for Flag-tagged HES1 or HEY2 were incubated with GST fusion proteins of HEY1 or HEY1-S68D coupled with Sepharose beads. The associated proteins were detected by western blotting using anti-Flag antibody. (B) Whole-cell extracts from U2OS cells previously transfected with expression vector for p53 were incubated with GST fusion proteins of HEY1, the mutant HEY1-S68A, or HEY1-S68D coupled with Sepharose beads. The associated proteins were detected by western blotting using anti-p53 antibody.