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. 2016 Jun 3;36(3):e00343. doi: 10.1042/BSR20160123

Figure 5. Simulation of HEY1 phosphorylation at residue Ser-68 inhibits HEY1 ability to induce p53-dependent cell cycle arrest.

Figure 5

(A) Immunoblot analysis of HEY1 expression in stable U2OS cell lines expressing inducible HEY1 (U2OS-HEY1) or the phosphomimetic mutant HEY1-S68D (U2OS-S68D) treated with 1 μg/ml tetracycline (Tet) for 4, 8 or 24 h. The protein levels of β-actin are shown as a loading control. (B) U2OS-HEY1 cells (top panel) or U2OS-S68D cells (bottom panel) were treated either with vehicle (Control) or 1 μg/ml tetracycline (Tet). Cell proliferation was monitored at different time points by using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The results shown represent the averages of results of two independent experiments assayed in quadruplicate ± S.D.