Figure 6. Expression of HEY1 but not HEY1-S68D leads to increased sensitivity to cisplatin, doxorubicin and resveratrol.

U2OS-HEY1 cells (left panels) or U2OS-S68D cells (right panels) were treated with 1 μg/ml tetracycline (Tet) to induce expression of HEY1 or HEY1-S68D or with vehicle (Control). After 8 h cells were cultured with varying concentrations of cisplatin (A), doxorubicin (B) or resveratrol (C) for another 72 h in the presence and absence of tetracycline. Subsequently, cell viability was assayed using an MTS-based assay. Data were plotted relative to the drug-free controls. The results shown represent the averages of results of two independent experiments assayed in quadruplicate ± S.D.