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Oxidation of biphenyl by P450 2A13 using analysis with HPLC with UV detection (A, B, and C) and fluorescence detection (F, G, and H). HPLC chromatograms of incubations of biphenyl (50 μM) with P450 2A13 (concentration of 0.025 μM) (A and F), 0.05 μM (B and G), and 0.1 μM (C and H), and chromatograms of standard 2-hydroxybiphenyl (2.5 nmol; D and I) and standard 4-hydroxybiphenyl (E and J). Detection was by UV absorbance (A–E) or fluorescence intensity (F–J).
