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Simulated and experimental thermodynamic stability. (A) Heat capacity curve of apo wild-type, apo Ser111Ala, and apo Ser111Leu systems was determined by replica-exchange molecular dynamics using 48 replicas ranging from 30 to 200 K, and the structure-based model of the stable structure [37, 38]; temperature is in reduced units. (B) Thermal stability of the different BLVRB variants as determined by shift assays from 20–70 °C.
