Fig. 7.

Initial rate kinetics of wild-type, Ser111Leu, and Ser111Ala BLVRB. Initial rates are plotted against the indicated concentrations of NADPH and delineated concentrations of FMN. Assays were performed in 100 mM potassium phosphate (pH 7.5) at 25 °C, and all data points represent the mean±standard error of the mean of triplicate determinations. The solid lines are least-squares fits to a rectangular hyperbola. Data are shown for the (left) wild-type (0.5 µg), (middle) Ser111Leu (2 µg) and (right) Ser111Ala (1 µg).