. Author manuscript; available in PMC: 2018 Feb 3.

Published in final edited form as: Chemistry. 2017 Jan 11;23(8):1891–1900. doi: 10.1002/chem.201604517

Tab. 1.

Serine111 mutation characteristics

Mutation

K_{m}^{F M N} (µ M)

K_{m}^{N A D P H} (µ M)

k_cat (s⁻¹)

k_{cat} / K_{m}^{N A D P H} (M^{- 1} \cdot s^{- 1})

Wild-type^¶

102 ± 47

8.3 ± 2.1

0.171 ± 0.03

2.1 ± 10⁴

S111L^¶

162 ± 21

5.8 ± 3.2

0.010 ± 0.001

1.7 ± 10³

S111A^¶

137 ± 13

6.7 ± 3.7

0.025 ± 0.005

3.7 ± 10³

^¶

All assays completed using identical reaction parameters at 25 °C. Errors represent standard deviation of the fit.