Table 2.
The release and recovery of captured H5N2 AIV from the pSiNW microdevice
| Initial volume | Diluted volume for RT- qPCR |
CT value of RT-qPCR |
Overall recovery efficiency |
|
|---|---|---|---|---|
| Original sample A | 120 µl | 120 µl | 15.92 | - |
| Recovered sample A1 | 60 µl | 60 µl | 17.43 | 17.6% |
| Recovered sample A2 | 60 µl | 60 µl | 16.33 | 37.6% |
| Recovered sample A3 | 60 µl | 60 µl | 16.61 | 31.0% |
| Original sample B | 40 µl | 40 µl | 15.92 | - |
| Recovered sample B | 400 µl | 400 µl | 21.6 | 29.2% |
| Original sample B | 40 µl | 250 µl | 19.40 | - |
| Recovered sample B | 1000 µl | 1000 µl | 23.55 | 28.2% |