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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Toxicology. 2016 Dec 31;378:17–24. doi: 10.1016/j.tox.2016.12.010

Figure 10. TCDD reduced CD34 expression in stromal cell-free cultures.

Figure 10

HSCs (104 cells/well) were treated with vehicle (VH) (0.02% DMSO) or TCDD (30 nM) on day 0 and cultured in stromal cell-free cultures (RPMI media with supplemented with cytokines and 20% v/v of conditioned media). The expression of CD34 on HSC-derived HSPCs was measured by flow cytometry. A) The percentage of CD34+ HSPCs. B) The average expression level of CD34 on CD34+ HSPCs, represented by MFI. Data are presented as mean ± SE of triplicate measurements. * p <0.05, **p <0.01, ***p <0.001, compared to the VH by a two way ANOVA with Bonferroni posttest. Data are representative of three independent experiments.