Figure 3.
Mass spectroscopy sequence coverage for FPtk1 (Ptk1:541–821). A. High confidence MS/MS data for 37 unique peptides comprising 87 exclusive unique and 394 total spectra were used to assign sequence coverage for 246 (blue upper case font) of 281 amino acids achieving 87.5% coverage. Non-detected residues are in lower case. MS/MS spectra for peptide in box is shown in (B). Phosphorylated residues are shown in red upper case, and bolded font. B. Example of MS/MS spectra used to assign site specific phosphorylation to MS/MS, in this case to tyrosine-775 (Y775) in the carbamidomethylated (c) tryptic peptide FTNMcLVLNDVSYSGSR.