Validation of gene fusions and SCNVs detected in routine FFPE samples. (A) EML4-ALK intrachromosomal fusion found in sample no. 32 (NSCLC, adenocarcinoma, H&E left). Confirmation of ALK overexpression due to this EML4-ALK gene fusion by IHC (right). (B) CD74-ROS1 fusion found in sample no. 25 (NSCLC, adenocarcinoma, H&E left). Confirmation of ROS1 overexpression due to this gene fusion between CD74 (Exon 6) and ROS1 (Exon 34) by IHC (right). (C) Intragenic MET fusion (Exon 13-Exon 15; MET “exon 14 skipping”) found in sample no. 29 (lung metastasis of CRC, H&E left). Confirmation of membranous MET overexpression due to this MET exon 14 mutation by IHC (right). (D) MYC amplification found in sample no. 27 (CRC, H&E left). The gene region 8q24.21 on chromosome 8 had a copy number of 25. Confirmation of MYC amplification by IHC (right).