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Methylation in benign urothelial tissue, LG BC, and HG BC tissue in EOMES, GP5, PAX6, TCF4, and ZSCAN12 genes measured by Illumina 450K array through TCGA. Mann-Whitney U test was used to compare methylation between benign urothelial tissue (n = 21), LG MIBC (n = 21), and HG MIBC (n = 21). Kruskal-Wallis test was used to compare methylation between LG and HG. *P < .05, **P < .01, and ***P < .001. + represents outliers.
