. 2017 Feb 7;8:159. doi: 10.3389/fmicb.2017.00159

Table 1.

Change of relative abundance (%) of bacterial phyla in four fractions during rumen development.

Fraction	Age (d)							SEM¹	Significance (P<)²
	1	10	20	38	41	50	60		Fraction	Age	Fraction × Age
PROTEOBACTERIA
RS	–³	–	8.49^c	1.33^b	10.79^b	11.67^b	3.71^b	0.810	<0.01	C (<0.05)	NS
RL	70.25^b	47.13^b	17.38^bc	2.17^b	19.62^ab	13.83^b	4.12^b			C (<0.05)
RP	–	–	25.80^ab	1.95^b	19.86^ab	10.33^b	1.20^b			L (<0.01)
RE	89.05^a	78.79^a	47.14^a	18.97^a	37.33^a	56.37^a	26.13^a			Q (<0.01)
FIRMICUTES
RS	–	–	47.07	66.73^a	35.51	41.85^a	55.70^b	0.574	<0.01	C (<0.05)	NS
RL	12.20	20.86^a	38.61	55.88^ab	44.47	41.06^a	61.69^ab			L (<0.01)
RP	–	–	46.48	63.33^a	35.53	46.87^a	73.44^a			NS
RE	6.65	8.59^b	36.97	43.02^b	39.61	23.55^b	44.86^b			Q (<0.01)
BACTEROIDETES
RS	–	–	27.37^ab	24.86	28.73^a	30.66^a	18.92^a	0.478	<0.01	NS	<0.05
RL	14.28^a	29.44^a	35.59^a	35.86	27.61^a	29.96^a	10.69^ab			Q (<0.01)
RP	–	–	19.34^bc	26.90	19.59^ab	25.52^a	6.20^b			Q (<0.05)
RE	2.31^b	6.27^b	11.76^c	26.82	10.31^b	11.88^b	15.55^ab			Q (<0.05)
FIBROBACTERES
RS	–	–	5.09	0.02	1.55	1.03	0.11	0.176	NS	NS	NS
RL	0.08^b	0.08	1.28	0.11	0.79	1.06	0.07			NS
RP	–	–	1.55	0.72	0.26	0.51	0.12			L (<0.05)
RE	0.82^a	0.31	1.54	0.15	0.62	0.17	0.13			L (<0.01)
SPIROCHAETAE
RS	–	–	7.02^a	1.69^a	5.48^a	4.53^a	2.97^a	0.070	<0.01	NS	<0.05
RL	0.02	0.31	1.52^b	0.71^ab	1.36^b	2.96^a	1.05^b			Q (<0.05)
RP	–	–	0.38^c	0.52^b	0.81^b	1.14^b	0.16^c			C (<0.01)
RE	0.28	0.27	0.84^bc	0.60^ab	1.13^b	1.23^b	2.88^a			L (<0.01)
ACTINOBACTERIA
RS	–	–	1.73	3.32	15.19^a	2.52	4.49	0.356	<0.01	NS	NS
RL	2.49	0.78	0.62	2.90	1.63^b	0.94	0.43			C (<0.05)
RP	–	–	2.21	5.71	22.40^a	3.87	1.10			Q (<0.05)
RE	0.44	2.96	1.24	8.68	9.36^a	2.28	3.49			Q (<0.05)
SYNERGISTETES
RS	–	–	1.54	1.01	0.66	6.90	13.62^ab	0.279	<0.01	Q (<0.01)	<0.05
RL	0.02	0.24	1.81	1.95	1.09	9.17	21.10^a			C (<0.01)
RP	–	–	2.89	0.51	0.55	10.94	17.52^a			Q (<0.01)
RE	0.31	0.12	0.21	1.26	0.56	4.28	6.63^b			Q (<0.01)

^{a, b, c}

Means within a column for fractions that do not have a common superscript differ (P <0.05).

SEM for fraction × age;

NS, not significant (P > 0.05); L, Linear effect of age; Q, Quadratic effect of age; C, Cubic effect of age;

No data due to the absence of samples.