Figure 3.

RacR directly regulates the dcuA and dcuB promoters. (A) Real-time RT-PCR data of the dcuA, dcuB, dcuC, dcuC2, and dctA genes in wild-type C. jejuni compared to the RacR mutant or the complemented RacR mutant strain. Data of four independent experiments with two independent preparations of RNA are presented as mean ds ± standard deviation, ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. (B) Electrophoretic mobility shift assays with the aspA, dcuB, dcuC, dcuC2, and dctA promoter regions labeled with [γ-³²P]ATP and phosphorylated RacR protein. Radioactive labeled promoter regions are marked with ^*. RacR was phosphorylated by RacScyto in the presence of ATP. The specificity of the protein-DNA interaction was determined by the addition of a 10-fold excess of unlabelled promoter region DNA.