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Addition of nitrate under limited oxygen conditions nullifies the C4-dicarboxylate mutant phenotype. Growth curves were generated of C. jejuni wild-type, the single mutants dcuA, dcuB, dcuC, dcuC2, and dctA, the double mutant dcuA/B and the triple mutant dcuA/B/C in HI with 25 mM nitrate and 25 mM fumarate (A) or aspartate (B) at 42°C under oxygen-limited conditions. The optical density at 600 nm was measured every 15 min. The experiments were repeated three times in duplicate.
