Table 1.
Bacterial strains and plasmids used in this study.
| Strains or plasmids | Genotype or relevant characteristics | Source or reference |
|---|---|---|
| STRAINS | ||
| C. jejuni 81116 | wildtype | Palmer et al., 1983 |
| dctA | 81116 derivative dctA::Cm | This study |
| dcuA | 81116 derivative dcuA::Cm | This study |
| dcuB | 81116 derivative dcuB::Km | This study |
| dcuC | 81116 derivative dcuC::SpeC | This study |
| dcuC2 | 81116 derivative dcuC2::Cm | This study |
| dcuAB | 81116 derivative dcuA::Cm dcuB::Km | This study |
| dcuABC | 81116 derivative dcuA::Cm dcuB::Km, dcuC::SpeC | This study |
| E. coli PC2955 | relA1 Φ80dlacZΔM15 phoA8 hsdR17 recA1 endA1 gyrA96 thi-1 relA1 luxS glnV44 | NCCB |
| PLASMIDS | ||
| pJet 1.2 blunt | ApR PCR cloning vector, Ampr | Fermentas, thermoscientific |
| pAV35 | ApR CmR pBluescript II SK containing Campylobacter coli CmR cassette | van Vliet et al., 1998 |
| pMW2 | ApR KmR pBluescript KS containing C. jejuni Kmr cassette | Wösten et al., 2010 |
| pZW2 | ApR SpecR E. coli-C. jejuni shuttle vector | Zhou et al., 2012 |
| pNBspec | ApR SpecR pAV35 containing SpecR cassette | This study |
| pJetdctA | ApR; 6.2 kb pJet containing dctA region | This study |
| pJetdcuA | ApR; 7.5 kb pJet containing dcuA region | This study |
| pJetdcuB | ApR; 6.3 kb pJet containing dcuB region | This study |
| pJetdcuC | ApR; 6.6 kb pJet containing dcuC region | This study |
| pJetdcuC2 | ||
| pJetdcuC2 | ApR; 8.7 kb pJet containing dcuC2 region | This study |
| pJetdctA::Cm | ApR CmR pJet containing dctA gene on a 2009 bp fragment | This study |
| pJetdcuA::Cm | ApR CmR; 5.9 kb, dcuA replaced by CmR | This study |
| pJetdcuB::Km | ApR KmR; 6.5 kb, dcuB replaced by KmR | This study |
| pJetdcuC::Spec | ApR SpecR; 6.2 kb, dcuC replaced by SpecR | This study |
| pJetdcuC2::Cm | ApR CmR; 8.5 Kb, dcuC2 replaced by CmR | This study |