Figure 5.

Effects of FeTPPS on NO production after TBI. Brain tissue section from sham mice (a) and sham + FeTPPS (b) haven't shown any positive staining for nitrotyrosine. Sections from TBI-injured mice demonstrated positive staining for nitrotyrosine (c), notably reduced by FeTPPS treatment (d). In addition, iNOS expression and plasma levels of nitrite/nitrate (NO₂) were also performed. At 24 h after trauma, an important increase in the iNOS expression (f) and plasma levels NO₂- were observed in brain (g) compared with the sham mice. FeTPPS treatment significantly reduced iNOS expression and NO₂ plasma levels. β-actin was used as internal control. A representative blot of lysates obtained from each group is shown, and densitometry analysis of all animals is reported (N = 10 mice from each group). ^***p < 0.001 vs. Sham + veh; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. TBI + veh; F 102.8.