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. 2017 Feb 7;7:27. doi: 10.3389/fcimb.2017.00027

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Copyright © 2017 Wang, Liu, Luo, Chen, Li, Su, Zhao, Liu, Xie, Jia, Ding, Wan and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MEK1 interacted with Ubc9 for SUMO1 conjugation. (A) Cells lysates were immunoprecipitated with anti-c-Myc antibody and immunoblotted with anti-HA or Myc antibody. (B) HEK293 cells were co-transfected with HA-MEK1 and one of the Flag-tagged SUMO isoforms. Immunoprecipitated HA-MEK1 was either blotted with an anti-HA antibody or was probed for SUMOylation using an anti-Flag antibody. SUMO1 modification of endogenous MEK1 was observed in cells (C,D).