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. 2017 Feb 7;8:34. doi: 10.3389/fphar.2017.00034

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Copyright © 2017 Saranya, Shilpa, Raghu and Priya.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Phase contrast image showing the detachment of cells under control/Morus alba leaf lectin (MLL) treated conditions in a time and concentration dependent manner. Magnification -4×. (B) MTT assay indicating the percentage of viable cells under control and varying concentrations of MLL for specific time intervals. Values are average of three independent experiments ± SD and ^∗p < 0.05 when compared to control. (C) Phase contrast image showing detached cells on cell culture plates coated different matrix proteins. The cells were not able to attach and grow further. But the trypsinised cells (control) seeded on matrix proteins were attached and grow further. Magnification -10×. (D) MTT assay showing the growth of cells after anoikis induction by MLL. Values are average of three independent experiments ± SD and ^∗p < 0.05 when compared to control.