Figure 13.

Evaluation of pro-fibrotic properties of splenic B cells in phosphate-buffered saline (PBS) and HOCl mice at day 21 and day 42. (A) Transforming growth factor (TGF)-β mRNA levels in splenic B cells after collection and sorting, normalized to GUSB and expressed as fold changes to PBS day 21 for day-21 groups (left column), and to PBS day 42 for day-42 groups (right column) (n = 4–6 per group). (B–E) mRNA levels of markers of fibrosis [α-smooth muscle actin (α-SMA) (B), collagen (C), and fibronectin (D)] and proliferation [proliferating cell nuclear antigen (PCNA) (E)] in 3T3 fibroblasts cultured alone (control; gray bars), with day-42 PBS B cells (white bars) or with day-42 HOCl B cells (black bars). Results normalized to GAPDH and expressed as fold changes to control (n = 5–8 per group).