Table 1.
Phenotypic definitions of spleen cell and B cell subsets.
| Cell subsets | Abbreviation | Phenotypic definition |
|---|---|---|
| Spleen cell subsets | ||
| B cell | / | CD19+ |
| T cell | / | CD3+ |
| T CD4+ cell | / | CD3+ CD4+ |
| T CD8+ cell | / | CD3+ CD8+ |
| NK cell | / | CD335+ |
| Monocyte–macrophage | MM | CD11b+ FSChi |
| B cell subsets | ||
| B2 cell | B2 | CD19+ B220hi |
| Immature B cells | ||
| =Transitional B cell | TR | CD19+ B220hi CD93+ |
| Type 1 transitional B cell | T1 | CD19+ B220hi CD93+ CD23− IgMhi |
| Type 2 transitional B cell | T2 | CD19+ B220hi CD93+ CD23+ IgMhi |
| Type 3 transitional B cell | T3 | CD19+ B220hi CD93+ CD23+ IgMlo |
| Marginal zone precursor B cell | MZP | CD19+ B220hi CD93−/+ CD23hi IgMhi CD21hi |
| Mature naive B cells | CD19+ B220hi CD93− | |
| Follicular B cell | FO | CD19+ B220hi CD93− CD23+ CD21lo/med |
| Follicular type I B cell | FO-I | CD19+ B220hi CD93− CD23+ CD21lo/med IgMlo |
| Follicular type II B cell | FO-II | CD19+ B220hi CD93− CD23+ CD21lo/med IgMhi |
| Marginal zone B cell | MZ | CD19+ B220hi CD93− CD23− CD21hi IgMhi |
| Nature non-naïve B cells | ||
| Germinal center B cells | GC | CD19+ CD38− GL7+ |
| Centroblasts | CB | CD19+ CD38− GL7+ CXCR4+ |
| Centrocytes | CC | CD19+ CD38− GL7+ CXCR4− |
| Antibody-secreting cells | ASC | CD138hi CD19lo/− |
| Plasmablasts | PB | CD138hi CD19lo CD22+ |
| Plasma cells | PC | CD138hi CD19lo/− CD22− |
| Memory B cells | MemB | CD19+ CD93− CD38+ IgM− |
| B1 cells | B1 | CD19+ B220lo CD23− CD43+ IgMhi |
| B1a B cells | B1a | CD19+ B220lo CD23− CD43+ IgMhi CD5+ |
| B1b B cells | B1b | CD19+ B220lo CD23− CD43+ IgMhi CD5− |
| Regulatory B cells | ||
| CD5+ CD1dhi B cells | / | CD19+ CD5+ CD1dhi |
| IL-10+ B cells | B10 | CD19+ IL-10+ |
CXCR, CXC-chemokine receptor; FSC, forward scatter; Ig, immunoglobulin; IL, interleukin.