Table I. Potential CADA-affected PowerBlot targets selected for validation.
| Uniprot ID | Protein | Function | CLa | Fold change PBb | Fold change WBd |
|---|---|---|---|---|---|
| Q9ULH1 | ASAP1 (Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1) | Regulates ADP-ribosylation factor 1 GTPase activity | 10 | − n.v.c | 1.1 |
| Q15283 | GAP1m (Ras GTPase-activating protein 2) | Inhibitory regulator of Ras cAMP signaling Binds inositol tetrakisphosphate (IP4) | 10 | −n.v. | 1.3 |
| P63208 | p19 skp1 (S-phase kinase-associated protein 1) | Component of the SCF ubiquitin ligase complex | 10 | −n.v. | 1.1 |
| P42224 | STAT1 (Signal transducer and activator of transcription 1) | Signal transducer and transcription activator for interferon and cytokine signaling | 10 | −5.5 | 1.2 |
| P35637 | FUS (RNA-binding protein FUS) | Binds DNA, may act as a transcription activator | 10 | −3.2 | −1.2 |
| Q13426 | XRCC4 (DNA repair protein XRCC4) | Involved in non-homologous end joining DNA repair | 10 | −n.v. | 1.2 |
| P30281 | Cyclin D3 (G1/S-specific cyclin-D3) | Regulates cell cycle progression | 10 | 5.7 | 1.2 |
| Q13501 | Sequestome-1 (p62 Lck ligand) | Phosphotyrosine-independent ligand for the Lck SH2 domain | 10 | 2.4 | 1.1 |
| Q08999 | pRb2 (Retinoblastoma-like protein 2) | Regulates cell cycle progression | 10 | 3.1 | 1.7* |
| P05412 | c-Jun (Transcription factor AP-1) | Transcription factor, binds AP-1 and CRE-like sites | 9 | −2.0 | −1.9* |
| Q99523 | Sortilin (Neurotensin receptor 3) | Sorting receptor in the Golgi compartment | 7 | −2.1 | −3.8* |
| P06239 | p56 Lck (Tyrosine-protein kinase Lck) | CD4/CD8 associated signal transducer for T-cell antigen receptor | 3 | −2.1 | 1.2 |
| P01730 | CD4 (cluster of differentiation 4) | MHC class-II antigen/TCR co-receptor | n.v. | n.v. | −8.2* |
a Confidence level: assigned using signal criteria listed in Table S1.
b Mean fold change of the nine comparisons between DMSO and CADA samples in a 3 × 3 matrix as explained in Materials and Methods.
c n.v. = no value. See the results section for details on CD4 detection in the PowerBlot system. Presence versus absence of a protein: fold change is not calculable. When no signal could be detected in the CADA (or DMSO) sample but sufficient signal in the corresponding DMSO (or CADA) sample, the difference was classified as > twofold, although no actual ratio could be calculated (number/0).
d Mean fold change in four classical Western blots, numbers significantly different from 1 are indicated with (*).