Figure 5.

Square fluctuations of Hb monomers and tetramers. (a) Square fluctuations of α₁ subunits in isolated and tetrameric states based on the first 20 ANM modes. The differences of mobilities at α₁-β₁ and α₁-β₂ interfaces are indicated by red and green circles. (b) Square fluctuations of β₁ subunits in isolated and tetrameric states based on the first 20 ANM modes. Red, green, and black circles indicate the differences of mobilities at α₁-β₁ and α₂-β₁ interfaces and a common region of these two interfaces. (c) Square fluctuations of T-Hb tetramers based on the highest two modes. Hot spots are predicted by the peaks in the profile, while α₁β₁ and α₂β₂ dimer show the same prediction result.