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. 2004 Oct 25;4:41. doi: 10.1186/1471-2180-4-41

Table 3.

Primer sequences, gene targets and expected product sizes for viruses on the molecular strip.

Name Sequence (5' to 3') Gene Target 1st/2nd Size (bp)
PF1 1A CCT TAA ATT CAG ATA TGT AT HN1 1 478
PF1 1B GAT AAA TAA TTA TTG ATA CG
PF1 1C CCG GTA ATT TCT CAT ACC TAT G 2 317
PF1 1D CCT TGG AGC CGA GTT GTT AAG
PF2 1A AAC AAT CTG CTG CAG CAT TT HN1 1 508
PF2 1B ATG TCA GAC AAT GGG CAA AT
PF2 1C CCA TTT ACC TAA GTG ATG GAA T 2 204
PF2 1D GCC CTG TTG TAT TTG GAA GAG A
PF3 2A CTTG TAA ACT CAG ACT TGG TA HN1 1 478
PF3 2B TTT AAG CCC TTG TCA ACA AC
PF3 2C ACT CCC AAA GTT GAT GAA AGA T 2 103
PF3 2D TAA ATC TTG TTG TTG AGA TTG
hMPV 1A GCG GCA ATT TTC AGA CAA CG Fusion 1 696
hMPV 1B ACA TGC TGT TCG CCT TCA AC
hMPV 1C CAG CAG CAG GAA TCA ATG TT 2 288
hMPV 1D TCG CCT TCA ACT TTG CTT AG
CoV229E 1A GGT ACT CCT AAG CCT TCT C G Nucleocapsid 1 450
CoV229E 1B TGC ACT AGG GTT AAG AAG AGG
CoV229E 1C TTT GGA AGT GCA GGT GTT GTG G 2 100
CoV229E 1D GAC TAT CAA ACA GCA TAG CAG C
ENT 2A TCC TCC GGC CCC TGA ATG 5' Non-coding 1 120
ENT 2B GAA ACA CGG ACA CCC AAA GTA
ENT 1C GGC CCC TGA ATG CGG CTA AT 2 90
HRV 1B GGT CCC RTC CCG CAA TT
FLA 3A (H1)2 GAA ATT TGC TAT GGC TGA C Haemagglutinin 1 482
FLA 3B (H1)2 ATA TTT TGG GCA CTC TCC TAT
FLA 3C (H1)2 GTC TCT GTA GTG TCT TCA CAT TAT 2 197
FLA 3D (H1)2 CCG GAC CCA AAC CCT CTA CTC
FLB 1A GTG ACT GGT GTG ATA CCA CT Haemagglutinin 1 900
FLB 1B TGT TTT CAC CCA TAT TGG GC
FLB 1C CAT TTT GCA AAT CTC AAA GG 2 767
FLB 1D TGG AGG CAA TCT GCT TCA CC
FLA 2C (H3)2 AGC AAA GCT TTC AGC AAC TG Haemagglutinin 1 591
FLA 2D (H3)2 GCT TCC ATT TGG AGT GAT GC-3
FLA 2E (H3)2 AGT GCT GAA CGT GAC TAT GC 2 149
FLA 2F (H3)2 TTT GCT GGC TTC TCT TGG T
RSV 2A GTC TTA CAG CCG TGA TTA GG Nucleoprotein 1 838
RSV 2B GGG CTT TCT TTG GTT ACT TC
RSVA 2C GAT GTT ACG GTG GGG AGT CT 2 334
RSVA 2D GTA CAC TGT AGT TAA TCA CA
RSVB 2C AAT GCT AAG ATG GGG AGTTC 2 183
RSVB 2D GAA ATT GAG TTA ATG ACA GC
ADV 2A2 GCCGCAGTGGTCTTACATGCACATC Hexon 1 301
ADV 2B2 CAGCACGCCGCGGATGTCAAAGT
ADV 2C32 GACGCCTCGGAGTACCTSWSYCC 2 185
ADV 2DD2 TACGAGTACGTGGTGTCCTCKCGRTC

1 HN = Haemagglutinin-neuraminidase gene; 2 Primer sets designed for this study. N.B. 1 = 1st round primer set, 2 = 2nd round primer set; ENT 2A 2B, 1C, HRV 1B primer set = Rhinovirus.