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. 2017 Feb 7;7:41364. doi: 10.1038/srep41364

Figure 7. The Smurf-10A mutation partially inhibits the Smurf activity towards BMP and TGFβ signaling and cell migration.

Figure 7

(A) HEK293T cells were transfected with of the indicated Smurf1 constructs and BRE-luc plasmids. Thirty-six hours after transfection, cells were treated with BMP-2 (200 ng/ml) for 12 h before BMP signaling activity was measured. Data are mean ± s.d. (n = 3). Asterisk means P < 0.05, student’s t-test. Western-blot analysis of whole-cell lysates with p-Smad1/5 or Smad1/5 antibody was also shown. (B) Transwell assay of the indicated HeLa cells. Data from three independent experiments were presented (mean ± S.D.) Asterisk means P < 0.05, student’s t-test. (C) HEK293T cells were transfected with of the indicated Smurf2 constructs and 3TP-luc plasmids. Thirty-six hours after transfection, the TGFβ signaling activity was measured. Western-blot analysis of whole-cell lysates with p-Smad3 or Smad3 antibody was also shown. Data are mean ± s.d. (n = 3). Asterisk means P < 0.05, student’s t-test.