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. 2017 Feb 7;7:42070. doi: 10.1038/srep42070

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(a) FACS plots of C6 cells treated and untreated with 5-ALA. The percentages of fluorescence(−) and (+) cells treated with 5-ALA are indicated in representative FACS plots (upper), and mean fluorescence intensities are presented in a bar graph (lower) as means ± SD from three independent experiments. *P < 0.05. (b) Detection of intracellular PpIX in 5-ALA-treated C6 cells by HPLC. Fluorescence(−) and (+) cells were sorted from C6 cells treated and untreated with 5-ALA, and analyzed by HPLC. Data are normalized by protein concentrations of lysates and presented in a bar graph as mean ± SD from three independent experiments. n.d., not detected.