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. 2017 Feb 7;7:42245. doi: 10.1038/srep42245

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Study design. Colonic contents from C57BL/6NTac donor mice were used for inoculating germ-free Tac:SW parental (P) mice by oral gavage. P mice were housed in a flexible film isolator or in individually ventilated cages (IVCs). Mice were bred to include P, F1 and F2 generations in the study. The figure is not reflecting the actual numbers of cages. (b) Timeline of breeding and fecal samplings. Boxes on the timeline illustrate when fecal samples for 16S rRNA gene sequencing were collected, e.g., P-7 = fecal sampling from P mice 7 weeks old. † = termination of group.