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. 2017 Feb 7;7:42144. doi: 10.1038/srep42144

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Copyright © 2017, The Author(s)

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CD4+ T cells were cultured under Th1 differentiation conditions and treated with DCPAB and HPAB for 5 days. Cells were restimulated with anti-CD3 for 24 h. (a) Scheme for Th1 cell differentiation. (b) Cells were harvested and incubated with PE-conjugated anti-IFN-γ Ab, followed by flow cytometry analysis. Cell populations were quantitated using CellQuest software. (c) Cell supernatants were collected from the differentiated Th1 cells and used for measuring IFN-γ by ELISA. (d) IFN-γ production was determined in DCPAB- and HPAB-treated cells by ELISA and real time-PCR. IFN-γ suppression activity of DCPAB and HPAB was comparatively analyzed and is expressed as the percentage of suppression activity normalized to the untreated control (100%). At least three independent experiments were performed and statistically analyzed. *P < 0.05, **P < 0.005, ***P < 0.0005.