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. 2017 Feb 7;7:42204. doi: 10.1038/srep42204

Figure 5. Remethylation of DNA from activated AC reduces IL-6 production and boosts TGFβ by DC and B cells in mice with inflammatory arthritis.

Figure 5

Mice were left untreated (UT) or injected with liposomes or liposomes loaded with DNA from activated AC (LaAC) or liposomes loaded with DNA from activated AC that had been re-methylated (LmaAC) at the time of immunization with CFA/mBSA and for a further 2 consecutive days. 14 days after immunization, the percentages of DC (CD11c) and B cells (CD19) in the draining lymph nodes producing TGFβ (A) and percentages of DC and B cells in the spleen producing IL-6 (B) were determined by flow cytometry. (C) Ratio of IL-6 to TGFβ production by DC and B cells. Graphs show mean ± SEM of combined data from 3 independent experiments with 9 mice per group. FACS plots show representative data. Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Bonferroni post hoc testing for multiple comparisons.