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. 2017 Feb 7;7:42204. doi: 10.1038/srep42204

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Copyright © 2017, The Author(s)

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(A) CD4⁺ T cells were isolated from PBMC of healthy controls and patients with RA and SLE and cultured overnight with etoposide to induce apoptosis. DNA was isolated from CD4⁺ AC and digested for the determination of global methylated cytidine levels by ELISA. Graphs show mean ± SEM. Healthy n = 6, RA n = 8, SLE n = 12. (B,C) The AC generated in (A) were cultured with monocyte-derived macrophages from healthy donors for 72 hours. Supernatants were assayed by ELISA for TGFβ and CBA for IL-6. (D) Ratio of IL-6 to TGFβ production by monocyte-derived macrophages. Graphs show mean cytokine production ± SEM. Statistical analysis was performed using a t-test.