Figure 9. Proposed mechanism by which n-3 and n-6 DPA inhibits SPC-induced Ca²⁺-sensitization of VSM contraction by inhibiting activation and translocation of Rho-kinase.

SPC stimulation causes SPC-induced Ca²⁺-sensitization and abnormal contraction of VSM via the SPC/SrcPTKs/Rho-kinase pathway. Additionally, SPC-induced translocation of Rho-kinase from cytosol to the cell membrane plays a vital role in SPC-induced contraction. Here, we demonstrated that n-3 and n-6 DPA inhibit SPC-induced Ca²⁺-sensitization of VSM contraction via inhibiting the activation and translocation of Rho-kinase.