Table 1.
Sequences of PCR primers used and their PCR conditions.
| Nucleotide change tested (gene) | Primers sequences (5'-3') | PCR conditions |
| C512T (EIF2B2), C547T (EIF2B2) | F: GCAAAACCGTTCTTAC R: CCTACCCATCTCTCGTTTAT |
PCR preparation: 1.5 mM MgCl2, 0.225 mM dNTP, 0.8 μM primers, 100 ng primers, 1 unit AmpliTaq Gold™(Applied Biosystems), 1X Taq buffer. |
| 607–612del/insTG (EIF2B2), A638G (EIF2B2) | F: GGAAATTATGTGCTGGATATG R: ACTTTATTCTCTCACCGTGGAT |
|
| P243L (EIF2B4) | F: ATGCTCAAGCTCCCTTTCAA R: CTTCACAACTTACAAAGCCT |
|
| R374C (EIF2B4) | F: ATTCAAGCACCTGGCATGAT R: CGCTGCACTCCATCCTTATC |
PCR reaction: 95°C 12 min, 35 cycles (94°C 30 s, 55°C 30 s, 72°C 45 s), 72°C 10 min, 4°C. |
| T1393C (EIF2B4), T1465C (EIF2B4) | F: TGTCCTGTAAGTAGGGGACCTT R: AAGGGGTTGTGAAGTCTGGA |
|
| G338A (EIF2B5), C583T (EIF2B5) | F: GAGAAGGACTGTGAGTGCTGA R: GCCTTCTAAGGGGACAATAAC |
F: forward primer, R: reverse primer