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. 2017 Feb 7;7:42125. doi: 10.1038/srep42125

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(A) Western blot analysis of EMT makers. (B) Immunofluorescence analysis of ZO1 and E-cadherin localization in NC and MCF10A-shDLG5 cells and of ZO1 and N-cadherin localization in NC and MCF7-shDLG5 cells. Scale bar, 25 μm. Monolayer wound healing assay (C,D: scale bar, 100 μm) and transwell assay (E,F: scale bar, 50 μm) of NC, MCF10A-shDLG5, MCF7-shDLG5 and MDA-MB-231-oxDLG5 cells.