Table 1. MALS-SEC data summarizing the molecular weight of the complex forming analysis for the different prenisin peptide variants and without any prenisin peptide.
| NisB | NisC | NisA variants | Molekular weight [kDa] |
|---|---|---|---|
| + | − | — | 237.5 ± 0.3 |
| + | − | unmodified | 241.9 ± 0.4 |
| − | + | — | 48.1 ± 0.5 |
| − | + | unmodified | 54.5 ± 0.6 |
| + | + | unmodified | 293.6 ± 1.2 |
| + | + | dehydrated | 291.2 ± 0.9 |
| + | + | modified | 250.4 ± 0.7 |
| + | + | FNLD-Box | 248.0 ± 0.9 |
The theoretical molecular weight of a NisB dimer is calculated to 236.6 kDa, 48.5 kDa for a cleaved NisC monomer and 5.9 kDa for the unmodified prenisin peptide.