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. 2017 Feb 7;7:41574. doi: 10.1038/srep41574

Figure 1. Collection of Angiostrongylus cantonensis excretory-secretory products (ESP) and induction of cell death in astrocytes.

Figure 1

(a) ESP were obtained from the living fifth-stage larvae cultured in RPMI medium for 24, 48, 72 and 96 h. SDS-PAGE was used to determine the protein concentrations of ESP. (b) Viability of astrocytes in co-cultures with different concentrations of ESP (0 to 500 μg/ml) for 2 h and at (c) different time points (0 to 8 h). Bovine serum albumin (BSA) and PBS were used as controls (white circles). Data are expressed as the means ± SD based on three independent experiments (**P < 0.01).