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. 2017 Feb 7;7:41574. doi: 10.1038/srep41574

Figure 2. Apoptosis of astrocytes induced by Angiostrongylus cantonensis excretory-secretory products (ESP).

Figure 2

(a) FASCan flow cytometric analysis of apoptosis of astrocytes co-cultured with or without 500 μg/ml ESP or BSA for 4 h (Annexin V−/PI−: normal cells, Annexin V+/PI−: early stage apoptotic cells, Annexin V+/PI+: late stage apoptotic cells, Annexin V−/PI+: necrosis cells). (b) Western blot analysis of Bax and Caspase-3 levels in astrocytes with and without treatment of 500 μg/ml ESP for 4 h. β-actin was used as a control. The full-length blots are presented in Supplementary Figures S1 and S2.