Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 7;7:42191. doi: 10.1038/srep42191

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

CD4⁺ T-cells were activated in the presence or absence (Medium) of the indicated concentrations of CQ and activated with anti-CD3/anti-CD28 antibodies or left unstimulated. After 48 hours, the extracellular acidification rate (ECAR; representing anaerobic glycolysis) and the oxygen consumption rate (OCR; representing mitochondrial respiration) were measured. (A) Mean ± SD from one representative donor (n = 5) (B) Mitochondrial stress test; At the indicated time points (arrows), the ATP coupling reagent oligomycin, the electron transfer chain uncoupling reagent FCCP, and the mitochondrial inhibitors rotenone/antimycin A were added. Data depict mean ± SD from quintuplet cultures from one representative donor (n = 5).